Journal: Measurement Science & Technology
Article Title: Viscoelastic recovery times of chondrocytes measured using a novel 3D-printed microfluidic device
doi: 10.1088/1361-6501/adf65a
Figure Lengend Snippet: Each Z -stack in the time-series is converted to a single image by a MIP 2D projection in Imaris. Then the time-series is imported into Matlab and the projected area of each cell is found by an edge detection algorithm. The time series shows a single cell with the edge marked in red at different time points, t 1, t 2, and t 3. Note that, for simplicity, only one cell is shown; however, there are more cells within the FOV.
Article Snippet: To calculate the total projected area (shadow) of the cells before, during, and after compression, each Z -stack of the time series was reduced to a single image using the maximum intensity projection (MIP) operation in Imaris (Oxford Instruments).
Techniques: